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Image 78 of Progress report (Kentucky Agricultural Experiment Station) n.176

Part of Kentucky Agricultural Experiment Station

-76- before treatment and 24 hours after treatment, were analyzed in triplicate for vitamin A E, chromic oxide, and polyethylene glycol. Percent disappearance of vitamin E was estimated from weighted averages of _ ratios of vitamin E to chromic oxide and polyethylene glycol in abomasal contents 24 hours after administering vitamin E. Results and Discussion Disappearance of vitamin E averaged 8.4, 22. 2, 25. 0, and 42.4% for the 20, 40, 60, and 80% rations, respectively. Except for the difference between the 40% and 60% rations, all differences between individual rations were significant (P ( .02). The data indicate that large portions of vitamin E administered orally to steers fail to reach . the small intestine. Adding grain to the ration appears to increase this disappearance. EFFECT OF CONCENTRATE LEVEL ON PRE-INTESTINAL 1 DISAPPEARANCE OF VITAMIN A R. L. Warner, G. E. Mitchell, Jr., C. O. Little, and N. E. Alderson Results of previous experiments have indicated that there may be considerable destruction of the ingested vitamin A before it reaches the small intestine. High con- centrate rations are most likely to require vitamin A supplementation. Since level of concentrate in the ration affects rumen fermentation, it seems possible that it could affect destruction of vitamin A in the rumen. This report gives the results of an experiment conducted to study this possibility. Procedure Four mature steers with permanent abomasal fistulas were fed four alfalfa, - corn, soybean meal rations, containing 20, 40, 60, and 80% corn and balanced for protein, calcium, phosphorus, and vitamin A, in ascending order and then in descend- ing order of corn content. Each steer was fed 2.5 kg twice daily. A 2-week preliminary period was followed by two recovery trials one week apart and then the rations were changed. Pre-intestinal disappearance of vitamin A was estimated by administering approximately 1, 000, 000 I.U. of vitamin A acetate dispersed in 19 ml of 20% aqueous tween "80", 20 grams of chromic oxide and 20,000 I.U. of vitamin E in gelatin capsules. 'I\ventyfour hours after dosing, abomasal contents were collected through the abomasal fistula and analyzed in triplicate for vitamin A and chromic oxide. Changes in the ratios of chromic oxide to vitamin A from the ratio administered were used to estimate percentages of administered vitamin A reaching the abomasum. Results and Discussion Calculated preintestinal disappearances of vitamin A averaged 52. 2% for the _ 20% corn ration, 55.9% for the 40% corn ration, 70. 1% for the 60% corn ration, and 62. 2% for the 80% corn ration. Disappearances in steers fed 20 or 40% corn were within ranges previously observed for all roughage rations and were not significantly

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