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Image 70 of Progress report (Kentucky Agricultural Experiment Station) n.176

Part of Kentucky Agricultural Experiment Station

-68- . concentrate mix free choice at a ratio of 6.3 to l. 0. Eighty percent of the Q libitum intake was fed during the balance and digestibility trials. The low sulfur group excreted significantly less sulfur in urine but not in feces _ or milk. All cows were in negative sulfur balance, but in positive nitrogen balance. The supplemental sulfur was inefficiently utilized, as indicated by the increased urinary o excretion of sulfur and by no effect on sulfur balance. Therefore, inorganic sulfur only partially meets the cow's total dietary sulfur requirement. There were no differences between groups in apparent digestibility of dry matter, crude fiber, crude protein, nitrogen free extract, ether extract, total digestible nutrients, or energy. Balance of amino acids was very similar for both groups. All ,_ animals were essentially in balance for methionine, cystine, and tyrosine, in negative balance for glutamic acid and lysine, and in positive balance for histidine, arginine, ` aspartic acid, threonine, serine, glycine, alanine, valine, isoleucine, leucine, phenylalanine, and proline. The data suggest that inadequate rumen synthesis of certain amino acids limits voluntary feed intake and milk production of high producing cows in which 45% of the nitrogen of the diet is nonprotein nitrogen. PROPERTIES OF TOXIC FESCUE EXTRACTS Suh-Jen Yau and D. R. Jacobson Eighty percent ethanol extract of toxic Kentucky 31 fescue was diluted with an V equal volume of water and dialyzed against water overnight at 40C. The dialysis extract was concentrated to the original volume and administered to two rats. Intraperitoneal - injections of the extract at 26.47g hay equivalent per 100g body weight increased tail skin temperature 3. 6OC after 21 min, which was followed by a decrease to below the I original. There was a 5. 30C reduction of deep body temperature at 1. 75 hours. Purple discoloration (the first sign of necrosis) was noted on the tail the 15th day after extract administration began. Purification of crude ethanol extract was also done by extraction with a water- ~ chloroform system. The physiologically active factor was always present in the aqueous phase. Intraperitoneal administration to rats gave a response similar to that of the dialysis extract. Occasionally twitching was observed. Death usually occurred after higher dose administration. The toxic principle was water extractable, non- volatile, and heat stable. Paper and thin layer chromatographic studies of the extract revealed properties of polybasic acids. ESTABLISHMENT OF MICROBIAL ACTIVITY IN PREWEANED LAMBS . S. E. Poe, G. E. Mitchell, Jr., P. G. Woolfolk, G. L. M. Chappell, and W. P. Deweese Previous trials indicated that lambs could be weaned at 4 weeks of age. The objective of this study was to measure quantitatively the microbial activity of young lambs receiving milk only and milk plus creep.

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