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Image 388 of Annual report. 1912

Part of Kentucky Agricultural Experiment Station

258 Bulletin N0. 165. ` ij., _ ` extremely sensitive, showin-gi typical symptoms of metritis, fr< Plates were made of the cotyledons of the afterbirth. Bits of cul the cotyledons were injected subcutaneously into a pregnant asw guinea pig. 'The pig died over night and upon being posted lui t i / was found to be infected with the colon bacillus. She had, thi 1 however, aborted one pig before dying. Although the cow se< e was given every attention, receiving daily vaginal douches, slie oti · 1 exhibited on the ninth day after calving symptoms of sep- asl 9 ticaemia, and on the 23rd day of July, two weeks after calving, be tf, died. The veterinarian posted the cow and pronounced her Hii death due to metroperitonitis, the organism causing her death Bi ` was found to be an invasion of the Bacillus pseudomonas _ pyocyaneus, Migula. ` Of Plate cultures of the afterbirth of this cow had been made I fd ` in the usual manner- After being incubated twenty-four hours hi aerobically most of them presented considerable growth-of h? colonies of foreign germs,ranging‘in size from a pin point to dt two millimeters in diameter. The clear places were marked . al and the plates incubated three days, Nowak method. After P a great deal of searching with acmicroscope a few very small SY · colonies of the Abortusbcallus, Bang, were found on a limited Di area of one of the plates. The control not growing well, it fi was thought that perhaps something was 'wrong with the “` ] incubation so the plates were returned to the jar and incu·bated Q? ifi as before for an additional three days. At the end of this fl fi time the colonies were no larger, and an examination revealed A T ii; the fact that there must have been some mistake in standard i d , izing the agar used in connection with the platings of the foetai ti = »·‘ membranes of the cow as it was found to be 1.5 per cent. acid P t ` to phenolphthalein, while the agar used in the platings of the 0 . contents of the internal organs of the foetus was only 0.4 p€1` Qi i cent. acid. Hencewe concluded the acidity of the agar had 3 ~ retarded the growth of the bacillus in the platings made f1`0m ` Sl ` the afterbirth. 0 On August 1st, 1911, cow No. 42, of Herd No. 1, aborted if a male foetus in the 192nd day of gestation. This cow ab01‘i€d ii in the pasture and the foetus was not discovered until the next A day. The foetus weighed 4,940 grams and measured two f€€i i ii

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